| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.109240 |
| Chromosome: | chromosome 1 |
| Location: | 5685214 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g040500 | (1 of 1) K10643 - CCR4-NOT transcription complex subunit 4 (CNOT4, NOT4, MOT2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGGCCACCACAATCACGAAATGCAGAGC |
| Internal bar code: | CTCTACGCATTGGGGCACCGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 666 |
| LEAP-Seq percent confirming: | 99.0052 |
| LEAP-Seq n confirming: | 2090 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGTGACTGTGATGTTGGG |
| Suggested primer 2: | TTCGAGAGCCAGCATTAGGT |