| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.109256 |
| Chromosome: | chromosome 2 |
| Location: | 5714842 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g110100 | MLO1 | (1 of 1) PTHR10891//PTHR31942//PTHR31942:SF1 - EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN // FAMILY NOT NAMED // MLO-LIKE PROTEIN 1; MLO family protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACGAAGAGGAGGAGGAGGACGGCAGCAAC |
| Internal bar code: | ATCGACGTGATGGCGGTCGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 396 |
| LEAP-Seq percent confirming: | 99.1051 |
| LEAP-Seq n confirming: | 443 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATGACTGCAGCCAAGAACG |
| Suggested primer 2: | CTCATCCTATCCGCCACACT |