Insertion junction: LMJ.RY0402.109262_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.109262
Chromosome:chromosome 9
Location:893351
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g401350 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTACCACCACACCACCGTCACCACCACGCC
Internal bar code:GTCGAGTTTGATTACTTGTTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:597
LEAP-Seq percent confirming:98.0057
LEAP-Seq n confirming:344
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GTTCTTCACATGCTGCTCCA
Suggested primer 2:TGACCCTGGTCTGGTGTGTA