Insertion junction: LMJ.RY0402.109262_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.109262
Chromosome:chromosome 12
Location:3337772
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre12.g497653 SNE13,NED1 (1 of 8) PTHR10366//PTHR10366:SF411 - NAD DEPENDENT EPIMERASE/DEHYDRATASE // HIGH CHLOROPHYLL FLUORESCENCE PHENOTYPE 173 PROTEIN; Putative Cinnamoyl-CoA reductase/flavanone 4-reductase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCACGGAAGCTCCTTAGCAGACTCTGTGC
Internal bar code:TCGAAGCGTGCCGCCGCAGAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:2907
LEAP-Seq percent confirming:99.005
LEAP-Seq n confirming:1194
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:TGAGTAGAAACCCTTGCGCT
Suggested primer 2:ATGCAGGTGTCCATCATCAA