Insertion junction: LMJ.RY0402.109263_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.109263
Chromosome:chromosome 12
Location:4412808
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre12.g520950 BOP5,IDA-IC138,IC138,DIC4 Axonemal inner dynein arm I1 intermediate chain; (1 of 1) PTHR12442:SF12 - WD REPEAT-CONTAINING PROTEIN 78 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCAGGTACTGCTCGCTGTACGACGTACTG
Internal bar code:GGGACGGCACCAATTCCCCTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:76
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:107
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CCACCTCACACTCAGCTCAA
Suggested primer 2:CGACTTCTCCACCTACTCGC