| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.109270 |
| Chromosome: | chromosome_3 |
| Location: | 2890837 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g162850 | FAP292 | Flagellar Associated Protein | sense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATCAAGCCATATCTAGCGCTCTATCAAAAG |
| Internal bar code: | GGTCCCCAGCGGGCCGCGGATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 306 |
| LEAP-Seq percent confirming: | 98.3287 |
| LEAP-Seq n confirming: | 353 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGCACGCATATTGTAGGG |
| Suggested primer 2: | GCGTCATGCAAAGAAGTGAA |