| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.109299 |
| Chromosome: | chromosome 12 |
| Location: | 6376045 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g537581 | (1 of 1) 3.5.4.10 - IMP cyclohydrolase / Inosinicase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAGCCTGCAGGGCGCCAGGCATTTCTTTC |
| Internal bar code: | TCTGGACCCTATCTTGGAGATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 233 |
| LEAP-Seq percent confirming: | 71.659 |
| LEAP-Seq n confirming: | 311 |
| LEAP-Seq n nonconfirming: | 123 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAAGCACGTCAAGTCCAAC |
| Suggested primer 2: | GCAAGAAACTACGGTTCGGA |