| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.109307 |
| Chromosome: | chromosome 8 |
| Location: | 4387244 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g381900 | (1 of 2) PTHR22763:SF71 - PROTEIN Y105C5B.11, ISOFORM B | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTAATTGGAATGCATATGCTTTGCAGCAG |
| Internal bar code: | CTGTCAAGACGTGACGTTCGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 455 |
| LEAP-Seq percent confirming: | 97.1863 |
| LEAP-Seq n confirming: | 1727 |
| LEAP-Seq n nonconfirming: | 50 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGCACCACCTACCCTTGT |
| Suggested primer 2: | GCTTCAGCTTCATCTCCTGC |