| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.109334 |
| Chromosome: | chromosome 6 |
| Location: | 5507465 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g284850 | (1 of 1) K03155 - timeless (TIMELESS) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAATGTTGCGCGCCTGCGGTGTGCTTGCTG |
| Internal bar code: | CGCTGAATCGGACGCATGTGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 100 |
| LEAP-Seq percent confirming: | 97.561 |
| LEAP-Seq n confirming: | 120 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTACCACACAGATGGGGCT |
| Suggested primer 2: | GTGGCATTGCATAACAATCG |