| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.109345 |
| Chromosome: | chromosome 13 |
| Location: | 2017314 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g576800 | PHX21,HIF1,HIF | (1 of 1) K09592 - hypoxia-inducible factor prolyl hydroxylase (EGLN, HPH); Cytosolic prolyl 4-hydroxylase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCGGGCAGGGTAGGGAGTGTGGGATGCAG |
| Internal bar code: | GATCGCTGCGACGTTCGTACCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 674 |
| LEAP-Seq percent confirming: | 92.8924 |
| LEAP-Seq n confirming: | 2261 |
| LEAP-Seq n nonconfirming: | 173 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTATACCGCTTTCCCAACCA |
| Suggested primer 2: | ATAAGGGTTGCAGTTGCAGG |