| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.109352 |
| Chromosome: | chromosome 3 |
| Location: | 5817054 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g189100 | (1 of 1) PTHR31606:SF1 - WBP2 ORTHOLOG, ISOFORM E | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCACAGGAAGGCAAAAGGTTTTCCTTTGG |
| Internal bar code: | TATGGGGACATAGCGCCGGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 584 |
| LEAP-Seq percent confirming: | 98.9396 |
| LEAP-Seq n confirming: | 2426 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTCGACAACCAGACCACA |
| Suggested primer 2: | AACAAGCTGTTTGCGTGATG |