Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.109363 |
Chromosome: | chromosome 1 |
Location: | 2031505 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre01.g010880 | (1 of 2) K18598 - echinoderm microtubule-associated protein-like 6 (EML6) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTACACACAACCATTCTTACACATAACA |
Internal bar code: | GGAATCCTCGGTGTGCTCAGGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 571 |
LEAP-Seq percent confirming: | 81.7508 |
LEAP-Seq n confirming: | 1214 |
LEAP-Seq n nonconfirming: | 271 |
LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGCCAGAACCACATCAAGTT |
Suggested primer 2: | CGGTCATTGGTAGGTATCCG |