| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.109365 |
| Chromosome: | chromosome 13 |
| Location: | 2998910 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g584200 | GRL1 | Glutaredoxin-like protein; (1 of 1) PF03595 - Voltage-dependent anion channel (SLAC1) | CDS/3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAGTAGTAGCGCTCCCACAGGATGTTGAC |
| Internal bar code: | TTGTGCAGTCGTAGTGGCAGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 682 |
| LEAP-Seq percent confirming: | 99.1119 |
| LEAP-Seq n confirming: | 1116 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGTCCCTTCTTGCTACGC |
| Suggested primer 2: | TATGTGTGGAGAGGGGGAAG |