Insertion junction: LMJ.RY0402.109386_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.109386
Chromosome:chromosome 13
Location:5111808
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre13.g607400 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGGGCGCAACAGTAGGTGCCGGGCTGGAA
Internal bar code:ATTGGCCACGACCGGTAAATCT

Confirmation - LEAP-Seq

LEAP-Seq distance:546
LEAP-Seq percent confirming:99.1602
LEAP-Seq n confirming:3306
LEAP-Seq n nonconfirming:28
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:AGATCTTGTAGGTGGGGCCT
Suggested primer 2:CTGTGTGACTTGGACACGCT