| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.109395 |
| Chromosome: | chromosome 4 |
| Location: | 2823478 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g224150 | (1 of 1) 2.7.1.30 - Glycerol kinase / Glycerokinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCTGCAGCCCTCAGGGCGAAGGTGGACGG |
| Internal bar code: | CGCCAGTGTCCTCAACCCATCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 709 |
| LEAP-Seq percent confirming: | 99.3693 |
| LEAP-Seq n confirming: | 2836 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGTGTTTCACATTGGCTG |
| Suggested primer 2: | CCCAGCACGACATGTATGAG |