| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.109428 |
| Chromosome: | chromosome 12 |
| Location: | 4605790 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g522650 | (1 of 2) 2.4.1.232 - Initiation-specific alpha-1,6-mannosyltransferase / Glycolipid 6-alpha-mannosyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAGCGTTGGTTGCGGGAGGAGACGCGGTC |
| Internal bar code: | GAGGGAACAGTGGAGCTAAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 766 |
| LEAP-Seq percent confirming: | 99.094 |
| LEAP-Seq n confirming: | 875 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTTGCGTCGTGTATGGTGT |
| Suggested primer 2: | GCGTTTTGGGTCTAGATTGC |