| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.109433 |
| Chromosome: | chromosome_17 |
| Location: | 6714297 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g744247 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGCCTGTTGTGCCTTGTTTTGCGGGGTACT |
| Internal bar code: | GTTTCAGTTAGATCGTACTCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 650 |
| LEAP-Seq percent confirming: | 95.7219 |
| LEAP-Seq n confirming: | 179 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATCAAAGCGGTGCACTGAG |
| Suggested primer 2: | GATTGTGTGCCGGAAGAGAT |