| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.109475 |
| Chromosome: | chromosome_2 |
| Location: | 5197066 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g105700 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGGACTGCTGCCCCCTGCTGTGGGCAAAGC |
| Internal bar code: | ACTCCCACGGCTAATCCTATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1046 |
| LEAP-Seq percent confirming: | 99.6241 |
| LEAP-Seq n confirming: | 265 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTTTCACAACCAGGGTTCC |
| Suggested primer 2: | GGCACGGGTATATTGTGTCC |