Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.109490 |
Chromosome: | chromosome 5 |
Location: | 3154385 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre05.g239450 | (1 of 3) K14165 - atypical dual specificity phosphatase [EC:3.1.3.16 3.1.3.48] (K14165) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCACACATGATTACTTGGAACCTGTCGG |
Internal bar code: | CGTGTTGCGTTTTGGCAGCCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 508 |
LEAP-Seq percent confirming: | 98.8418 |
LEAP-Seq n confirming: | 8193 |
LEAP-Seq n nonconfirming: | 96 |
LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACCAGGACATACTTGGGCTG |
Suggested primer 2: | CAAGGCATGGGAATGCTTAT |