| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.109516 |
| Chromosome: | chromosome 3 |
| Location: | 2011954 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g156000 | (1 of 8) IPR014710 - RmlC-like jelly roll fold | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCCGCCGCCGCCGCCGCTGCCGGGGCCG |
| Internal bar code: | GCGGACGTGGTGCTGACCCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 42 |
| LEAP-Seq percent confirming: | 26.3514 |
| LEAP-Seq n confirming: | 39 |
| LEAP-Seq n nonconfirming: | 109 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCATGGACAGTCGACACG |
| Suggested primer 2: | GCATAGACGCCGTTAGCTTC |