| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.109556 |
| Chromosome: | chromosome 12 |
| Location: | 4222090 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g518800 | (1 of 2) PTHR11132:SF126 - PROTEIN C29A12.6, ISOFORM B | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACTCCTTCCCAGGGTATCGGCCTCTTAGC |
| Internal bar code: | CGGGTGGTAGTGCGCAGACGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 620 |
| LEAP-Seq percent confirming: | 99.2363 |
| LEAP-Seq n confirming: | 2469 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTTGCATGGACTTTACCG |
| Suggested primer 2: | GCGCCGATTGTACCATAGTT |