| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.109692 |
| Chromosome: | chromosome 3 |
| Location: | 8066712 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g201200 | FAP283 | Flagellar Associated Protein 283 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCACATCAATGATGAGAGGCCGATGGGTAA |
| Internal bar code: | GTATTCTCTGTTCGAATGAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 113 |
| LEAP-Seq percent confirming: | 99.0251 |
| LEAP-Seq n confirming: | 3555 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGAACTTGACCATAGGGCG |
| Suggested primer 2: | ATAACTCCCGTCCTGCACAC |