| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.109720 |
| Chromosome: | chromosome 1 |
| Location: | 7000208 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g050600 | (1 of 4) 2.1.1.62 - mRNA (2'-O-methyladenosine-N(6)-)-methyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGATAGGGAGACGGGAGGGTCGGATATG |
| Internal bar code: | CGAAAGCGCGGACGGTTACAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1084 |
| LEAP-Seq percent confirming: | 99.5784 |
| LEAP-Seq n confirming: | 4724 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGGGAGGGGGAGATATAA |
| Suggested primer 2: | GTCGGGAGAGAACGACAGAG |