| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.109724 |
| Chromosome: | scaffold 19 |
| Location: | 43449 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre19.g750447 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAGCCATGCCGCGGGTGGCAGGAACGACA |
| Internal bar code: | GCGGCAGACTCAGCAAGGCTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 301 |
| LEAP-Seq percent confirming: | 99.3773 |
| LEAP-Seq n confirming: | 798 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCGTAACGTGTGTGCGATA |
| Suggested primer 2: | GGCTAGGTAGCGACGACTTG |