| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.109725 |
| Chromosome: | chromosome 5 |
| Location: | 1201378 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g245350 | (1 of 1) IPR003582//IPR009011 - ShKT domain // Mannose-6-phosphate receptor binding domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGGACTGGGGGCGAGCGGTTGCGTGTGT |
| Internal bar code: | TCCTGAATGAGAAATGCGCAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 317 |
| LEAP-Seq percent confirming: | 97.9136 |
| LEAP-Seq n confirming: | 1314 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCGTCCTAGCTTGGTTTG |
| Suggested primer 2: | TTTTCATCCTTTCCCTGCTG |