| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.109728 |
| Chromosome: | chromosome_12 |
| Location: | 5290221 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g528850 | DLX1,DLC3 | Flagellar outer dynein arm 16 kDa light chain, LC3 | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AATCTCCACCACTGCAAATGTGCCCCCACA |
| Internal bar code: | TGGCAGCCGCTTCTTGTAAGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 916 |
| LEAP-Seq percent confirming: | 98.0108 |
| LEAP-Seq n confirming: | 2365 |
| LEAP-Seq n nonconfirming: | 48 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCCTGAGCATACCCCACAG |
| Suggested primer 2: | ACAGCTTTCGTCTCCTGCAT |