| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.109730 |
| Chromosome: | chromosome 3 |
| Location: | 7665985 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g204000 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTCTGGCGGAGCACATTCTGCCCTGACTG |
| Internal bar code: | ACTCGGTTCTGTGCTCTCTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 290 |
| LEAP-Seq percent confirming: | 81.7448 |
| LEAP-Seq n confirming: | 9220 |
| LEAP-Seq n nonconfirming: | 2059 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACACACACACACACACGG |
| Suggested primer 2: | CCAGCTCTTGAAAACGGAAG |