| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.109763 |
| Chromosome: | chromosome 2 |
| Location: | 5239159 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g106100 | (1 of 1) IPR000104//IPR029058//IPR029059 - Antifreeze protein, type I // Alpha/Beta hydrolase fold // Alpha/beta hydrolase fold-5 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTTAACGCGCGTTTGGGTCCGCGCTTTC |
| Internal bar code: | AGTGCTTGCGTCTCCATAGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 196 |
| LEAP-Seq percent confirming: | 93.617 |
| LEAP-Seq n confirming: | 220 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGGTGGATGTGAAGCTCT |
| Suggested primer 2: | CAAAATTGGTTCTGCACACG |