| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.109821 |
| Chromosome: | chromosome 10 |
| Location: | 3509420 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g445150 | EXN12 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTTGGGTTTGGGTTTGTACCTCCCGCCCA |
| Internal bar code: | ACGCTTTCGTCGTGTAGGTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 593 |
| LEAP-Seq percent confirming: | 99.0139 |
| LEAP-Seq n confirming: | 2912 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATGCGAATTTGCTTGCTTG |
| Suggested primer 2: | ATGCGTAATAACGTGCACCA |