| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.109827 |
| Chromosome: | chromosome_9 |
| Location: | 3708823 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g390949 | FAP140 | Flagellar Associated Protein | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCATGGTAGGCGGCGGCGCCACGACCCTAA |
| Internal bar code: | GAACAGTACATGTCAGAACACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 316 |
| LEAP-Seq percent confirming: | 90.9871 |
| LEAP-Seq n confirming: | 424 |
| LEAP-Seq n nonconfirming: | 42 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGCTTACCGAGTCTCCTG |
| Suggested primer 2: | CTATGGAGCAGTGTCTGGCA |