| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.109829 |
| Chromosome: | chromosome 16 |
| Location: | 6312726 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g674500 | ABCA4 | ABC transporter 4; (1 of 14) 3.6.3.25 - Sulfate-transporting ATPase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCAGGGCCTGAGGAGGACGGGGGTTTGCG |
| Internal bar code: | GCGGGTGAGGCATTTGCTTGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1159 |
| LEAP-Seq percent confirming: | 87.4764 |
| LEAP-Seq n confirming: | 7865 |
| LEAP-Seq n nonconfirming: | 1126 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCTGTTGGAAAACTGGAAA |
| Suggested primer 2: | TGTGCATTAGCTATGCGGAG |