| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.109874 |
| Chromosome: | chromosome 16 |
| Location: | 5983095 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g676650 | AP1G1 | Gamma1-Adaptin; (1 of 1) K12391 - AP-1 complex subunit gamma-1 (AP1G1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTACCATACTGTGATGGCTCGGCGTCTTC |
| Internal bar code: | CTCCGCACCTTAAGCTCTGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 635 |
| LEAP-Seq percent confirming: | 97.3924 |
| LEAP-Seq n confirming: | 6200 |
| LEAP-Seq n nonconfirming: | 166 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACTGCATCTCTCGACCGTG |
| Suggested primer 2: | GAGGGAATAGCCCGAATAGC |