| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.109886 |
| Chromosome: | chromosome 7 |
| Location: | 5935418 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g354250 | PGK2 | (1 of 2) PF00162 - Phosphoglycerate kinase (PGK); Phosphoglycerate kinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTCTGTGCAGCAGTCCAGTGTGAGCACC |
| Internal bar code: | GTTTGGTCTGACCGCTTAAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 0 |
| LEAP-Seq percent confirming: | 99.516 |
| LEAP-Seq n confirming: | 1028 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTGACGGCTTATGCAAAC |
| Suggested primer 2: | CTCCGATGATGGTAATGGCT |