| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.109888 |
| Chromosome: | chromosome 1 |
| Location: | 997794 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g005600 | FDX8 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGGCCCAGGTAGGCCCGGGCAGTCCGCC |
| Internal bar code: | TTTGGTCTGGGAGCCGTCGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1147 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 185 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGCGGTAGAGATCGAGTG |
| Suggested primer 2: | CCTAGTGCTTCCTCACCTGC |