| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.109895 |
| Chromosome: | chromosome 13 |
| Location: | 2703891 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g582201 | (1 of 1) PTHR13622//PTHR13622:SF8 - THIAMIN PYROPHOSPHOKINASE // THIAMIN PYROPHOSPHOKINASE 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTTGGAGAGATGCTGGGCGGGTGTGTGT |
| Internal bar code: | GTCAGCTGACAGGATGAAGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 502 |
| LEAP-Seq percent confirming: | 96.4332 |
| LEAP-Seq n confirming: | 3758 |
| LEAP-Seq n nonconfirming: | 139 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTTTGCAAGAACGTAGCC |
| Suggested primer 2: | GCCTGTTAACGCCAAGAGAG |