| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.109896 |
| Chromosome: | chromosome 12 |
| Location: | 6823784 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g561350 | (1 of 1) PTHR13244//PTHR13244:SF8 - ZINC FINGER MYND DOMAIN CONTAINING PROTEIN 10 // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTTACTGGTTCTGCGTTTTAGCAGGAAC |
| Internal bar code: | ACCATGTGCACCCATCACCCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1047 |
| LEAP-Seq percent confirming: | 99.6385 |
| LEAP-Seq n confirming: | 7167 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACAGAAGCGAAGGCATGTA |
| Suggested primer 2: | GGTGTCTACGCAGATGCTGA |