| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.109902 |
| Chromosome: | chromosome_3 |
| Location: | 6759433 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g198200 | BGS4,GTR13,GTR11 | 1,3-beta-D-glucan synthase | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCGCGTTGGCCACACGCTCACGGAAACTGT |
| Internal bar code: | CAGGCAGTAGCAGAACGTGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 729 |
| LEAP-Seq percent confirming: | 96.8877 |
| LEAP-Seq n confirming: | 716 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAGGTTGATGTGCAAAGGC |
| Suggested primer 2: | GTCGTCCTTCTCCGACTCAG |