| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.109905 |
| Chromosome: | chromosome 1 |
| Location: | 6853729 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g049500 | COX2B | Cytochrome c oxidase subunit II, protein IIb of split subunit | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGACGGCGTTGGAACCCCATACGCCTGCCT |
| Internal bar code: | TGGTCACGGAAATACCGCGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 581 |
| LEAP-Seq percent confirming: | 99.6124 |
| LEAP-Seq n confirming: | 514 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGGTACTGTAACACGCTGA |
| Suggested primer 2: | GTCTTCTACGGCCAGTGCTC |