Insertion junction: LMJ.RY0402.109922_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.109922
Chromosome:chromosome 6
Location:3757318
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g278156 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCGAGCGCTCCACATATGGCGTGAGACCG
Internal bar code:GCAAAATCGATTGATCACGGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:530
LEAP-Seq percent confirming:99.4028
LEAP-Seq n confirming:2663
LEAP-Seq n nonconfirming:16
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:CTGAAGGTGCTCTCAAAGGG
Suggested primer 2:AGTCAAAGGTGTGTGGAGGG