Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.109943 |
Chromosome: | chromosome 6 |
Location: | 2795363 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre06.g271150 | FAP74,C1d-HC3 | (1 of 6) PF14874 - Flagellar-associated PapD-like (PapD-like); Flagellar Associated Protein 74 | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGACGGAATGTACTTAATGCTCGCATTCG |
Internal bar code: | GCAGAGGGAGCCGTCCGGCCAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 506 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 44 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AAAAGACACCCATCGTCTGC |
Suggested primer 2: | GTATCCACTGCTGGTCGGTT |