| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.109952 |
| Chromosome: | chromosome 2 |
| Location: | 5535733 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g108550 | AOC3 | (1 of 4) PTHR11785//PTHR11785:SF367 - AMINO ACID TRANSPORTER // AMINO-ACID PERMEASE BAT1; Amino acid carrier | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTCCCAGAACCTCAACTACGCCGGTGAGT |
| Internal bar code: | CTGACAGTTTTAGTGCGTCACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 758 |
| LEAP-Seq percent confirming: | 67.1553 |
| LEAP-Seq n confirming: | 4169 |
| LEAP-Seq n nonconfirming: | 2039 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCCATTCTGCATTTCGTT |
| Suggested primer 2: | GTACCACACGCCACAAACAC |