| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.109957 |
| Chromosome: | chromosome 5 |
| Location: | 592870 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g232150 | GDH2 | Glutamate dehydrogenase; (1 of 1) 1.4.1.2//1.4.1.3 - Glutamate dehydrogenase / Glutamic dehydrogenase // Glutamate dehydrogenase (NAD(P)(+)) / Glutamic dehydrogenase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCAATGCTTGGATCCCGAGGATAGAAGC |
| Internal bar code: | TCATGTGGTTACTGGTTCCATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 128 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 149 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCCCCAAGACTTTTTCTTC |
| Suggested primer 2: | GAAGAAGCTGTAAGGCACCG |