| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.109977 |
| Chromosome: | chromosome 1 |
| Location: | 7379921 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g053050 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTAGCTATGCAGGGATTCCGCTAAAAAAT |
| Internal bar code: | CAAATGATATCTTTCTTTACTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 459 |
| LEAP-Seq percent confirming: | 51.8317 |
| LEAP-Seq n confirming: | 2292 |
| LEAP-Seq n nonconfirming: | 2130 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTCGCCTTAGCCACTACC |
| Suggested primer 2: | CCGTGATGAACTGTTGGTTG |