| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.109986 |
| Chromosome: | chromosome 2 |
| Location: | 2504809 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g092150 | CSL | (1 of 1) PTHR23155//PTHR23155:SF444 - LEUCINE-RICH REPEAT-CONTAINING PROTEIN // SUBFAMILY NOT NAMED; Chlamydomonas SHOC2/SUR8-like LRR | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTATTCGGGAAAATGTCGGGGTTCGGCG |
| Internal bar code: | TCAGTCAGCTGAGCACCCATTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 950 |
| LEAP-Seq percent confirming: | 99.6613 |
| LEAP-Seq n confirming: | 2060 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGACATGCGATTACACGGA |
| Suggested primer 2: | CCGAATACCGTATGCTTGCT |