| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.109990 |
| Chromosome: | chromosome 9 |
| Location: | 1657765 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g397050 | (1 of 28) PTHR31600:SF2 - TINY MACROCYSTS PROTEIN B-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGCCATTCCGCATGCGGCTACAGCAGCG |
| Internal bar code: | ACGACGCTATCCGCGAGAAGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 283 |
| LEAP-Seq percent confirming: | 98.8304 |
| LEAP-Seq n confirming: | 676 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTACCACCGTTTGCCTAC |
| Suggested primer 2: | CACCTCTTGGAAAGCTCCAG |