Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.109991 |
Chromosome: | chromosome 16 |
Location: | 5856915 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre16.g677600 | SOM5 | S-adenosyl-L-methionine-dependent O-methyltransferase; (1 of 4) PTHR13600//PTHR13600:SF12 - LEUCINE CARBOXYL METHYLTRANSFERASE // LEUCINE CARBOXYL METHYLTRANSFERASE | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGTCGCAACCAACATGGCCCCACTCCCA |
Internal bar code: | GTAGCGGGCGCTAGCGGCGACC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 531 |
LEAP-Seq percent confirming: | 55.641 |
LEAP-Seq n confirming: | 217 |
LEAP-Seq n nonconfirming: | 173 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCGAGAGTCGCAAATAGTCC |
Suggested primer 2: | CTTGCCCTCAGCCTTCATAG |