Insertion junction: LMJ.RY0402.109991_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.109991
Chromosome:chromosome 16
Location:5856915
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre16.g677600 SOM5 S-adenosyl-L-methionine-dependent O-methyltransferase; (1 of 4) PTHR13600//PTHR13600:SF12 - LEUCINE CARBOXYL METHYLTRANSFERASE // LEUCINE CARBOXYL METHYLTRANSFERASE intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGGTCGCAACCAACATGGCCCCACTCCCA
Internal bar code:GTAGCGGGCGCTAGCGGCGACC

Confirmation - LEAP-Seq

LEAP-Seq distance:531
LEAP-Seq percent confirming:55.641
LEAP-Seq n confirming:217
LEAP-Seq n nonconfirming:173
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GCGAGAGTCGCAAATAGTCC
Suggested primer 2:CTTGCCCTCAGCCTTCATAG