| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.110014 |
| Chromosome: | chromosome 7 |
| Location: | 2618258 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g330300 | (1 of 2) K14411 - RNA-binding protein Musashi (MSI) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACACAGTGCATTGGCCAATAGGGCAGGAC |
| Internal bar code: | CGAAGAGGCTCAGTCGCGGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 579 |
| LEAP-Seq percent confirming: | 98.9206 |
| LEAP-Seq n confirming: | 1558 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAACTCGCTCAACAACCAG |
| Suggested primer 2: | TCTTAGAGGTGCACAACCCC |