| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.110059 |
| Chromosome: | chromosome 8 |
| Location: | 1145721 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g362700 | SMM30 | (1 of 8) 3.4.24.71 - Endothelin-converting enzyme 1 / ECE-1; S-adenosyl-L-methionine-dependent methyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTTGTTGCAAGTACGGTATGGTGTGCGG |
| Internal bar code: | TCTAGACGGACGTGAGTCCCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 649 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 43 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCATGCGTAATCCTCTCAG |
| Suggested primer 2: | AGGAAGTAGGAGAGCGGAGG |