Insertion junction: LMJ.RY0402.110060_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.110060
Chromosome:chromosome 10
Location:5471714
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre10.g458850 SRR32 Scavenger receptor cysteine rich (SRCR) protein; (1 of 20) IPR001190//IPR017448 - SRCR domain // SRCR-like domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATTTATGATGTGGCAAACCGGCATTGAGCG
Internal bar code:CAACCGACGAGGCATACGGATA

Confirmation - LEAP-Seq

LEAP-Seq distance:520
LEAP-Seq percent confirming:99.7949
LEAP-Seq n confirming:2919
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CTGTCATCCGACACTCGCTA
Suggested primer 2:GAGGCAGGTCCACACTCATT