| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.110104 |
| Chromosome: | chromosome 3 |
| Location: | 1736052 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g153700 | FAP347 | Flagellar Associated Protein 347 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTCTTGTGTCCTAACCACTAAAACTGCG |
| Internal bar code: | GTGTTGAGCGAAGTTCGGGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 579 |
| LEAP-Seq percent confirming: | 98.3209 |
| LEAP-Seq n confirming: | 1581 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGTAGCCAATAGCCCAAT |
| Suggested primer 2: | AAGGTTCACAAACGGACCAC |